 | | Research activities
I have continued my work on biosensors since
2000 when I started my Ph.D. on development of clinical and environmental
sensors at Bhabha Atomic Research Centre, Mumbai, India and had a close
collaboration with Indian Institute of Technology, Bombay. During my brief
postdoc tenure at Indian Institute of Technology, Bombay and in about two
years as postdoc at Myongji University, South Korea I was engaged in
designing portable biosensors for clinical applications, while leading a
research team comprising of Ph.D. and undergraduate students. In the span of
time I successfully completed a number of academic interest projects on
design and development of biosensors (mainly standalone bench-top
prototypes) and finding new immobilization matrices and stabilization of
biomaterials therein. Those projects include:
•Development of integrated microfluidic lab-on-a-chip device for cell lysis,
PCR and capillary electrophoresis amperometric detection of PCR amplicon.
•Development of capillary electrophoresis amperometric detectors against
dopamine, catechol and endocrine disruptors.
•Development of PDMS based micropump and microvalve for integration onto PCR
lab-on-a-chip.
•Development of optical (methyl parathion), potentiometric (urea),
amperometric (glucose and catalase), conductometric (urea) and capacitive (creatine
kinase -MB) biosensors.
•Projects related to immobilization and stabilization of enzymes and
microbial cells in electrochemically and radiation polymerized synthetic
polymers such as polyaniline, polypyrrole, polyHEMA and copolymers of
polyacrylamide and polyvinyl alcohol.
•Interfacing of analogue electronics to digital environment using LabView
based virtual instruments for performing signal acquisition, conditioning
and analysis.
•Fabrication of computer controlled dissolved oxygen meter, sensitive to
parts per trillion, for assay of catalase in pathological samples, cell and
tissue preparations and for developing multi-purpose benchtop glucometer.
•Fabrication of silicon based interdigitated capacitive biosensor (creatine
kinase -MB).
•Development of computer controlled pH meter, sensitive to ninth decimal of
pH, for standalone potentiometric clinical urea biosensor.
A number of these works have already found place in SCI publications and
remainder of them are under circulation or preparation.
I also have worked on routine molecular biology; clinical biochemistry and
microbiological analysis techniques; isolation and maintenance of all cell
types including microbial, animal cell lines and plant tissue cultures and
also production, characterization and purification of enzymes. Apart from
them I am basically a chemist (First class Honours graduate from University
of Calcutta) and hence possess strong analytical skills which were evident
from my experience with polymer and enzyme chemistry.
I worked under guidance of following eminent scientists
Prof. Yong-Sang Kim, Department of Nanoscience and Engineering, Myongji University, Yongin,
South Korea
Prof. Dr. S. F. D'Souza, Associate Director, Bioscience Group, Bhabha Atomic Research Center, Trombay, Mumbai, 400085, E-mail: sfdsouza
[at] barc.gov.in Phone: +91-22-2559-3632
Prof. A. Q. Contractor, Head, Department of Chemistry, Indian Institute of Technology, Powai, Mumbai, 400076, E-mail: aqcontractor
[at] iitb.ac.in Phone: +91-22-2576-7151
Dr. S. K. Apte, Associate Director (B), Bioscience Group, Bhabha Atomic Research Centre, Trombay, Mumbai, Maharashtra, India. 400085.
E-mail: aptesk [at] barc.gov.in Phone: +91-22-25595342
Prof. V. R. Rao,
Department of Electrical Engineering, Indian Institute of Technology Bombay,
Mumbai, India
Prof. S.
Mukherji, Associate
Professor, Biomedical Engineering Group, Indian Institute of Technology, Powai, Mumbai, 400076, E-mail:
mukherji [at] iitb.ac.in Phone: +91-22-2576-7767
Dr. (Mrs.) Anita Topkar, Scientist, Electronics Division, Bhabha Atomic Research Center, Trombay, Mumbai, 400085, E-mail: anita
[at] barc.gov.in Phone: +91-22-7652-2059
Dr. (Mrs.) Aruna Bhatia, Head, Department of Biotechnology, Punjabi University, Patiala, Punjab
Prof. Chanchal Mitra, Department of Chemistry, Hyderabad Central University, Hyderabad, Andhra Pradesh
Prof. Pinaki Sar, Department of Biotechnology, Indian Institute of Technology, Kharagpur, West Bengal
And of course with many other scientists and engineers, who happens to be my
colleagues and mentor and guide on several occasions.
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